6TQ0

Crystal structure of the human Arc N-lobe bound to repeat peptide 5 from GKAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	39.310, 192.300, 39.560
Unit cell angles	90.00, 94.80, 90.00

Refinement procedure

Resolution	100.000 - 1.950
R-factor	0.2229
Rwork	0.221
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tno
RMSD bond length	0.012
RMSD bond angle	0.754
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.000
High resolution limit [Å]	1.950	1.950
Rmeas	0.053	2.073
Number of reflections	41702	3069
<I/σ(I)>	13.8	0.9
Completeness [%]	98.2	97.3
Redundancy	3.7
CC(1/2)	0.999	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277	1.2 M sodium citrate