6TPV

Crystal structures of FNIII domain one and two of the human leucocyte common antigen-related protein, LAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	63.270, 50.843, 65.525
Unit cell angles	90.00, 97.22, 90.00

Refinement procedure

Resolution	40.050 - 1.800
Rwork	0.185
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dju
RMSD bond length	0.014
RMSD bond angle	1.183
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (v1.16-3549-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.050	1.860
High resolution limit [Å]	1.800	1.800
Rmeas		0.980
Number of reflections	37701	2761
<I/σ(I)>	17.3	1.81
Completeness [%]	97.8	97.4
Redundancy	6.8	7.1
CC(1/2)	0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	277	0.2 M Sodium citrate tribasic dihydrate pH 8.3, 20 % PEG 3,350