6TPU

Crystal structures of FNIII domain three and four of the human leucocyte common antigen-related protein, LAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	85.290, 105.100, 62.000
Unit cell angles	90.00, 135.56, 90.00

Refinement procedure

Resolution	43.410 - 1.550
R-factor	0.1664
Rwork	0.165
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2edx
RMSD bond length	0.010
RMSD bond angle	1.131
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.410	1.600
High resolution limit [Å]	1.550	1.550
Number of reflections	54988	5296
<I/σ(I)>	14.4
Completeness [%]	98.6
Redundancy	5.1
CC(1/2)	0.999	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	1.8 M Ammonium citrate tribasic pH 7.0