6TP1
Crystal structure of Bacillus paralicheniformis alpha-amylase in complex with maltotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 82.080, 82.080, 186.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.260 - 1.940 |
| R-factor | 0.1866 |
| Rwork | 0.187 |
| R-free | 0.20100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6toy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.624 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.260 | 1.970 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.190 | 0.958 |
| Rmeas | 0.203 | 1.033 |
| Rpim | 0.071 | 0.378 |
| Number of reflections | 48251 | 2575 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 98.6 | 79.9 |
| Redundancy | 8 | 7 |
| CC(1/2) | 0.994 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 42 - 46 % Tacsimate, 10 mM CaCl2 |
