6TOQ
Crystal structure of a PP2A B56y/HTLV-1 integrase complex
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2017-07-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9192 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.770, 58.770, 321.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.290 - 3.164 |
| Rwork | 0.227 |
| R-free | 0.29410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jak |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.655 |
| Data reduction software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.290 | 3.380 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.167 | 0.489 |
| Rpim | 0.167 | |
| Number of reflections | 10041 | 1694 |
| <I/σ(I)> | 3.3 | 1.5 |
| Completeness [%] | 96.7 | 94.4 |
| Redundancy | 1.4 | |
| CC(1/2) | 0.918 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293.15 | 0.1 M Na/KPO4 pH 6.2, 20-% 1,2-propanediol, 10% glycerol |
