6TNK
CARBOXYPEPTIDASE T WITH N-SULFAMOYL-L-VALINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 157.531, 157.531, 104.523 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 1.900 |
| R-factor | 0.143 |
| Rwork | 0.142 |
| R-free | 0.15570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qnv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.845 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.980 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.144 | 0.330 |
| Rmeas | 0.148 | |
| Number of reflections | 60407 | 8678 |
| <I/σ(I)> | 15.1556 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.49 | 18.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 293 | 1.4 SA |
