6TNJ
Crystal structure of the vWF domain of the type V pili tip protein Mfa5 from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2017-09-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.54003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.198, 110.080, 38.178 |
| Unit cell angles | 90.00, 98.11, 90.00 |
Refinement procedure
| Resolution | 37.800 - 1.850 |
| R-factor | 0.1356 |
| Rwork | 0.132 |
| R-free | 0.17760 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.029 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.850 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.087 | 0.369 |
| Rmeas | 0.087 | 0.373 |
| Number of reflections | 23968 | 1123 |
| <I/σ(I)> | 75.6 | 24 |
| Completeness [%] | 97.6 | 75.9 |
| Redundancy | 171.8 | 109.8 |
| CC(1/2) | 1.000 | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20% PEG500, 10% PEG 20k, 0.1M Sodium Hepes / MOPS pH7.5, 0.1M Glu/Ala/Gly/Lys/Ser |
