6TMT
Crystal structure of the chaperonin gp146 from the bacteriophage EL 2 (Pseudomonas aeruginosa) in presence of ATP-BeFx, crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 137.920, 149.427, 268.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 4.030 |
| R-factor | 0.2583 |
| Rwork | 0.256 |
| R-free | 0.29910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tmu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.067 |
| Data reduction software | XDS (VERSION Jan 26, 2018) |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | MOLREP (11.4.06) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.980 | 48.980 | 4.170 |
| High resolution limit [Å] | 4.030 | 15.610 | 4.030 |
| Rmerge | 0.344 | 0.063 | 1.891 |
| Rmeas | 0.359 | 0.067 | 1.964 |
| Rpim | 0.099 | 0.021 | 0.528 |
| Total number of observations | 602390 | 8978 | 62099 |
| Number of reflections | 46685 | 888 | 4519 |
| <I/σ(I)> | 6.9 | 22.2 | 1.6 |
| Completeness [%] | 99.9 | 97 | 100 |
| Redundancy | 12.9 | 10.1 | 13.7 |
| CC(1/2) | 0.994 | 0.997 | 0.661 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 5 % PEG-4000, 0.2 M Na-acetate and 0.1 M Na3-citrate pH 5.5 |
