6TMR

Mokola virus glycoprotein, monomeric post-fusion conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.648727
Spacegroup name	C 2 2 21
Unit cell lengths	76.279, 81.951, 231.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.225 - 2.893
R-factor	0.229
Rwork	0.226
R-free	0.27830
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.225	3.070
High resolution limit [Å]	2.893	2.893
Rmeas	0.135
Number of reflections	31149	4917
<I/σ(I)>	10.16	0.95
Completeness [%]	99.6
Redundancy	6.92
CC(1/2)	0.998	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 4000, sodium acetate, hepes