6TMD

Crystal structure of KANK2 ankyrin repeats mutant (A670V)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.915870
Spacegroup name	C 1 2 1
Unit cell lengths	95.778, 45.659, 51.073
Unit cell angles	90.00, 100.38, 90.00

Refinement procedure

Resolution	47.150 - 1.500
R-factor	0.1788
Rwork	0.177
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hbd
RMSD bond length	0.009
RMSD bond angle	1.565
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.150	1.521
High resolution limit [Å]	1.500	1.500
Rmerge	0.056	2.239
Rmeas	0.067	2.674
Rpim	0.036	1.450
Number of reflections	35000	1775
<I/σ(I)>	10.3	0.5
Completeness [%]	99.2	99.9
Redundancy	3.4	3.2
CC(1/2)	0.999	0.329

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	294	0.2 M ammonium acetate, 0.1 M Bis-tris pH 5.7, 31% w/v PEG 3350