6TLZ

N-Domain P40/P90 Mycoplasma pneumoniae complexed with 3'SL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-09-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	C 1 2 1
Unit cell lengths	117.212, 107.312, 162.013
Unit cell angles	90.00, 90.12, 90.00

Refinement procedure

Resolution	81.010 - 3.100
R-factor	0.245
Rwork	0.244
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rj1
RMSD bond length	0.008
RMSD bond angle	1.030
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.010	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.123	1.062
Rmeas	0.149	1.281
Rpim	0.083	0.706
Number of reflections	35571	5211
<I/σ(I)>	5.3	1
Completeness [%]	97.4
Redundancy	3.1
CC(1/2)	0.989	0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	PEG 3350 and MetOH