6TLH

Crystal structure of mouse KANK3 ankyrin repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	C 1 2 1
Unit cell lengths	149.437, 45.778, 69.596
Unit cell angles	90.00, 95.06, 90.00

Refinement procedure

Resolution	53.170 - 1.800
R-factor	0.2012
Rwork	0.199
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hbd
RMSD bond length	0.012
RMSD bond angle	1.529
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.320	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.062	0.776
Rmeas	0.073	0.909
Rpim	0.039	0.472
Number of reflections	42604	2516
<I/σ(I)>	10.5	1.5
Completeness [%]	97.4	96.7
Redundancy	3.5	3.7
CC(1/2)	0.999	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2 M ammonium formate, 20% w/v PEG 3350