6TL4

Photosensory module (PAS-GAF-PHY) of Glycine max phyB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 31 2 1
Unit cell lengths	102.269, 102.269, 222.918
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.170 - 2.900
R-factor	0.2582
Rwork	0.257
R-free	0.28240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4our
RMSD bond length	0.009
RMSD bond angle	1.392
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.170	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.238	5.838
Rmeas	0.251	6.140
Rpim	0.077	1.893
Number of reflections	30704	4420
<I/σ(I)>	12.1	0.8
Completeness [%]	99.9	99.9
Redundancy	19.8	19.9
CC(1/2)	0.999	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	283.15	200mM MES, 200mM KSCN, 200mM LiCl2, 1% w/v gamma-PGA, 3% w/v PEG3350