6TKK

Neuropilin 1-b1 domain in a complex with the C-terminal VEGFB186 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	38.880, 39.980, 97.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.805 - 1.060
R-factor	0.1367
Rwork	0.136
R-free	0.14950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rn5
RMSD bond length	0.012
RMSD bond angle	1.299
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.810	48.810	1.090
High resolution limit [Å]	1.060	4.620	1.060
Number of reflections	55937	972	645
<I/σ(I)>	29.4
Completeness [%]	80.2
Redundancy	5.5
CC(1/2)	0.999	0.997	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	16 % w/v PEG3350 and 200 mM ammonium chloride