6TJR
Structure of HdrA-like subunit from Hyphomicrobium denitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 145.620, 145.620, 64.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.670 - 1.430 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.17900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 1.481 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmeas | 0.049 | 0.124 |
| Number of reflections | 138112 | 10500 |
| <I/σ(I)> | 20.1 | 1 |
| Completeness [%] | 96.7 | 74.02 |
| Redundancy | 5.8 | 2.4 |
| CC(1/2) | 1.000 | 0.403 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | MES 0.1M, PEP 629 25% (w/v) |
