6TJ7

T. gondii myosin A trimeric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.032
Spacegroup name	I 21 21 21
Unit cell lengths	84.630, 93.482, 108.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.960 - 2.300
R-factor	0.1881
Rwork	0.186
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tgh
RMSD bond length	0.007
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.740	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	1.010
Rmeas	0.080	1.100
Rpim	0.040	0.430
Number of reflections	19409	12474
<I/σ(I)>	13.83	1.79
Completeness [%]	99.8	99.9
Redundancy	6.4	6.6
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292.15	imidazole, MES monohydrate, PEG 500 MME, PEG 20000, 1-6-hexadiol, 1-butanol, 1,2-propanediol, 2-propnaol, 1,4-butandiol, 1,3-propanediol