6TJ6

T. gondii myosin A trimeric complex with ELC1, calcium-free

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 41
Unit cell lengths	86.831, 86.831, 54.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.900 - 2.000
R-factor	0.1913
Rwork	0.190
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vt9
RMSD bond length	0.003
RMSD bond angle	0.595
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.900	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.040	1.350
Rmeas	0.150	1.410
Rpim	0.010	0.390
Number of reflections	27763	2711
<I/σ(I)>	31	1.74
Completeness [%]	99.9	99.38
Redundancy	13.5	12.9
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292.15	PEG 8000, Tris, Lithium Chloride