6TIF

ReoM- Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976250
Spacegroup name	P 21 21 21
Unit cell lengths	38.788, 58.619, 74.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.252 - 1.600
Rwork	0.153
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5us5
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.450	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	23059	1129
<I/σ(I)>	8.2	2.2
Completeness [%]	99.8
Redundancy	4.8
CC(1/2)	0.986	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.1 M phosphate/citrate 0.2 lithium sulphate 20 % w/v PEG 1000