6TGZ
IE1 from human cytomegalovirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 2.033 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 109.993, 134.125, 70.541 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.604 - 3.200 |
R-factor | 0.2294 |
Rwork | 0.225 |
R-free | 0.26830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wid |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MR-Rosetta |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.604 | 48.604 | 3.280 |
High resolution limit [Å] | 3.200 | 14.310 | 3.200 |
Rmerge | 0.215 | ||
Rmeas | 0.224 | 0.064 | 1.567 |
Number of reflections | 8867 | 72 | 633 |
<I/σ(I)> | 7.73 | 28.63 | 0.99 |
Completeness [%] | 99.4 | 58.5 | 99.8 |
Redundancy | 12.946 | 12.222 | 12.607 |
CC(1/2) | 0.998 | 0.999 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | Na-malonate, PEG 3350 |