6TGK

Domain swapped E6AP C-lobe dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-03-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	I 4 2 2
Unit cell lengths	72.280, 72.280, 98.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.030 - 1.300
R-factor	0.1795
Rwork	0.178
R-free	0.20680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d5f
RMSD bond length	0.014
RMSD bond angle	1.877
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.030	1.347
High resolution limit [Å]	1.300	1.300
Rmerge	0.013	0.502
Rmeas	0.018	0.710
Rpim	0.013	0.502
Number of reflections	32542	3211
<I/σ(I)>	26.56	1.49
Completeness [%]	99.9	99.63
Redundancy	2	2
CC(1/2)	1.000	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1 M CaCl 0.1 M Sodiumacetat 30% PEG400