6TGK
Domain swapped E6AP C-lobe dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-03-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 72.280, 72.280, 98.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.030 - 1.300 |
| R-factor | 0.1795 |
| Rwork | 0.178 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d5f |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.877 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.030 | 1.347 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.013 | 0.502 |
| Rmeas | 0.018 | 0.710 |
| Rpim | 0.013 | 0.502 |
| Number of reflections | 32542 | 3211 |
| <I/σ(I)> | 26.56 | 1.49 |
| Completeness [%] | 99.9 | 99.63 |
| Redundancy | 2 | 2 |
| CC(1/2) | 1.000 | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M CaCl 0.1 M Sodiumacetat 30% PEG400 |
