6TGJ

RNA-binding domain of RNase M5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	27.801, 72.594, 72.985
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.492 - 1.500
R-factor	0.2121
Rwork	0.210
R-free	0.25230
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.492	1.551
High resolution limit [Å]	1.497	1.497
Rmerge	0.046	1.431
Number of reflections	24152	2249
<I/σ(I)>	20.16	1.14
Completeness [%]	98.1
Redundancy	7
CC(1/2)	0.999	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M cacodylate pH 6.5, 0.2 ammonium sulfate and 30% PEG 8000