6TG3
Crystal Structure of the PBP/SBP MotA in complex with glucopinic acid from A. tumefaciens B6/R10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.630, 131.930, 70.380 |
| Unit cell angles | 90.00, 91.71, 90.00 |
Refinement procedure
| Resolution | 48.120 - 1.850 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l9g |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.084 | 1.143 |
| Rpim | 0.034 | 0.462 |
| Number of reflections | 106626 | 16773 |
| <I/σ(I)> | 12.42 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.999 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | PEG 4K, 100 mM Tris HCl, 200 mM CaCl2 |
