6TG2
Structure of the PBP/SBP MotA in complex with mannopinic acid from A.tumefacien R10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-04 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.920, 132.130, 69.460 |
| Unit cell angles | 90.00, 91.23, 90.00 |
Refinement procedure
| Resolution | 31.190 - 2.210 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l9g |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.135 | 0.907 |
| Rpim | 0.093 | |
| Number of reflections | 60300 | 9388 |
| <I/σ(I)> | 6.31 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3 | |
| CC(1/2) | 0.990 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | PEG 4K, 100 mM Tris HCl |
