6TFM
Frizzled8 CRD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.296, 66.039, 72.666 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 72.666 - 2.343 |
| R-factor | 0.246 |
| Rwork | 0.243 |
| R-free | 0.30040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ijy |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_3488) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.700 | 2.380 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.089 | |
| Number of reflections | 20741 | 1024 |
| <I/σ(I)> | 7.3 | 2.3 |
| Completeness [%] | 98.9 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 300 | 0.1 M sodium acetate, pH 5 1 M ammonium sulphate |
