6TES

Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Cocrystal with Fe2+, Mn2+, UDP-Glucuronic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	98.202, 100.505, 224.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.100 - 2.200
R-factor	0.1928
Rwork	0.190
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fxk
RMSD bond length	0.007
RMSD bond angle	0.939
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.104	2.566
Rpim	0.060	1.554
Number of reflections	56604	4537
<I/σ(I)>	11.8	0.5
Completeness [%]	99.8	98.6
Redundancy	7.6	7.1
CC(1/2)	0.999	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH, 500 uM FeCl2, 500 uM MnCl2, 1 mM UDP-Glucuronic Acid