6TCC

Crystal structure of Salmo salar RidA-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.65
Spacegroup name	H 3 2
Unit cell lengths	52.189, 52.189, 242.565
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.070 - 1.050
R-factor	0.1458
Rwork	0.145
R-free	0.16330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oni
RMSD bond length	0.009
RMSD bond angle	1.061
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.350	1.110
High resolution limit [Å]	1.050	1.050
Number of reflections	60156	8683
<I/σ(I)>	21.1
Completeness [%]	100.0
Redundancy	19.4
CC(1/2)	1.000	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Sodium chloride, 0.2 M Sodium acetate pH 5, 20% (w/v) PEG 6000