6TC9
Crystal structure of MutM from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-12-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9795, 1.0389 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.770, 80.380, 83.620 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 36.223 - 2.175 |
| R-factor | 0.2401 |
| Rwork | 0.238 |
| R-free | 0.28090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tc6 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.223 | 2.253 |
| High resolution limit [Å] | 2.175 | 2.175 |
| Rmerge | 0.072 | 0.605 |
| Rmeas | 0.083 | 0.712 |
| Number of reflections | 44999 | 4371 |
| <I/σ(I)> | 7.63 | 1.95 |
| Completeness [%] | 98.6 | 95.2 |
| Redundancy | 3.9 | 3.3 |
| CC(1/2) | 0.997 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 100 mM Tris-HCl pH = 8.5, 200 mM Magnesium acetate, |
