6TC5
Phytochromobilin-adducted PAS-GAF bidomain of Sorghum bicolor phyB
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 133.875, 133.875, 46.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.820 - 2.100 |
| Rwork | 0.197 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tby |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.870 |
| Data reduction software | XDS (Mar 15 2019) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.820 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.151 | 1.620 |
| Rmeas | 0.175 | 1.873 |
| Rpim | 0.088 | 0.933 |
| Number of reflections | 28169 | 2275 |
| <I/σ(I)> | 10.7 | 1.3 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 7.4 | 7.7 |
| CC(1/2) | 0.997 | 0.536 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 283.15 | 100 mM Tris/HCl pH 8.5, 500 mM NaCl, 9% w/v PEG4000 |
