6TBT
Crystal structure of the BCL6 BTB domain in complex with an Apt48 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.630, 65.630, 156.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.840 - 1.630 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.17750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r2b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.331 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.200 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.112 | 1.770 |
| Rpim | 0.027 | 0.403 |
| Number of reflections | 49659 | 3390 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.39 | 19.98 |
| CC(1/2) | 0.999 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.4 M NaCl, 0.1 M Tris pH 7, 0.2 M MgCl2 |
