6TBD
Crystal structure of the SVS_A2 protein from ancestral sequence reconstruction at 2.30 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.300, 105.481, 105.481 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.200 - 2.300 |
| R-factor | 0.1856 |
| Rwork | 0.184 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4okz |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.580 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.139 | 0.677 |
| Rmeas | 0.172 | 0.853 |
| Rpim | 0.101 | 0.496 |
| Number of reflections | 37596 | 3635 |
| <I/σ(I)> | 9 | 2.9 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 5.2 | 5.2 |
| CC(1/2) | 0.991 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M BTP pH 6.5 0.2 M Na-Phosphate 20% PEG 3350 |
