6TA9
Bd0314 DslA wild-type form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97597 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.696, 47.818, 49.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.348 - 1.361 |
| R-factor | 0.142 |
| Rwork | 0.140 |
| R-free | 0.17970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EtoQmutant |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.029 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.380 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Number of reflections | 42769 | 1766 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 98.1 | |
| Redundancy | 12.4 | |
| CC(1/2) | 0.998 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1M Acetate pH 5.5 50% PEG 400 |
