6T8M

Prolyl Hydroxylase (PHD) involved in hypoxia sensing by Dictyostelium discoideum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976230
Spacegroup name	C 2 2 21
Unit cell lengths	45.216, 81.781, 379.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.920 - 2.020
R-factor	0.2291
Rwork	0.228
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j25 5l9r
RMSD bond length	0.002
RMSD bond angle	0.455
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	189.535	42.119	2.130
High resolution limit [Å]	2.020	6.390	2.020
Rmerge		0.037	1.426
Rmeas	0.110	0.040	1.512
Rpim	0.036	0.014	0.495
Total number of observations	429431	13255	61814
Number of reflections	47025	1666	6792
<I/σ(I)>	12.2	40.7	1.7
Completeness [%]	100.0	99.8	100
Redundancy	9.1	8	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M HEPES pH 7.5, 17.5-23% PEG 6000, 0.2M NaCl, 2mM NiCl2, 0-6% glycerol