6T84
crystal structure of the mycobacterial trehalose monomycolate transport factor A, TtfA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.240, 70.090, 86.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.540 - 1.400 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.19850 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.233 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.540 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.051 | 1.118 |
| Rmeas | 0.057 | |
| Rpim | 0.026 | |
| Number of reflections | 37320 | 3670 |
| <I/σ(I)> | 14.79 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.990 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 28% PEG 4000 and 0.2 M (NH4)2SO4 |
