6T7Y
Structure of PCNA bound to cPIP motif of DP2 from P. abyssi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 |
| Unit cell lengths | 140.736, 140.736, 38.521 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.370 - 2.700 |
| R-factor | 0.1929 |
| Rwork | 0.186 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5auj |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.138 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.370 | 2.863 |
| High resolution limit [Å] | 2.700 | 2.701 |
| Number of reflections | 6983 | 436 |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 89.5 | |
| Redundancy | 5.2 | |
| CC(1/2) | 0.999 | 0.210 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 30% PEG 400 0.2 M MgCl2 0.1M BIS-TRIS pH7.1 |
