6T7X

Crystal structure of PCNA from P. abyssi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9786
Spacegroup name	P 63
Unit cell lengths	91.902, 91.902, 64.188
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.204
Rwork	0.202
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5auj
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.480
High resolution limit [Å]	2.300	2.300
Number of reflections	13821	1387
<I/σ(I)>	14.02
Completeness [%]	99.8
Redundancy	14
CC(1/2)	0.990	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	20% PEG 400 0.2 M CaCl2 0.1 M MES pH 5.5