6T75
Bacteroides salyersiae GH164 beta-mannosidase 2-deoxy-2-fluoro-beta-D-mannosyl enzyme intermediate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.155, 104.873, 171.627 |
| Unit cell angles | 91.98, 97.73, 107.16 |
Refinement procedure
| Resolution | 89.337 - 2.550 |
| Rwork | 0.214 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t50 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.548 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.900 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 148606 | 7334 |
| <I/σ(I)> | 6.1 | 1 |
| Completeness [%] | 98.7 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.994 | 0.458 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Ammonium tartrate, PEG 3350 |
