6T6I
Crystal Structure of the C-terminal domain of the HIV-1 Integrase (subtype A2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 49.528, 49.528, 42.016 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.893 - 2.203 |
| R-factor | 0.2215 |
| Rwork | 0.218 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t6e |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3311) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.893 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.033 | |
| Number of reflections | 3045 | 295 |
| <I/σ(I)> | 57.69 | |
| Completeness [%] | 99.9 | |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 1M sodium malonate |
