6T69

Crystal structure of Toxoplasma gondii Morn1(V shape)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 62 2 2
Unit cell lengths	205.858, 205.858, 40.584
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.450 - 2.500
Rwork	0.206
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6t4d
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (phenix-1.17-3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.450	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.198	2.116
Number of reflections	18162	1960
<I/σ(I)>	14.6
Completeness [%]	99.7
Redundancy	17.9
CC(1/2)	0.999	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	0.1 M Tris-HCl pH 8.2, 15% PEG 3350, 0.2 M NaCl