6T68

Crystal structure of Trypanosoma brucei Morn1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.89
Spacegroup name	C 1 2 1
Unit cell lengths	192.878, 49.737, 41.984
Unit cell angles	90.00, 97.54, 90.00

Refinement procedure

Resolution	48.140 - 2.540
R-factor	0.2375
Rwork	0.234
R-free	0.29340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6t4d
RMSD bond length	0.004
RMSD bond angle	0.697
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.140	2.650
High resolution limit [Å]	2.530	2.530
Rmerge	0.116	1.365
Rpim	0.074
Number of reflections	12419	975
<I/σ(I)>	9.3
Completeness [%]	93.1	60
Redundancy	6.2
CC(1/2)	0.995	0.303

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.166 M Tris-HCl pH 8.8, 0.15 M MgCl2, 0.45 M KI, 24% PEG 2000 MME, and 4% glycerol