6T66

Crystal structure of the Vibrio cholerae replicative helicase (DnaB) with GDP-AlF4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	118.978, 123.292, 263.493
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.630 - 3.900
R-factor	0.256
Rwork	0.255
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qel
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	MOLREP (Vers 11.7.01)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.630	49.620	4.070
High resolution limit [Å]	3.900	17.650	3.900
Rmerge	0.226	0.080	3.860
Rmeas	0.293	0.083
Rpim	0.055	0.022	1.009
Number of reflections	38678	440	2494
<I/σ(I)>	6.2
Completeness [%]	99.8	94.9	97.7
Redundancy	18.3	15.7	15.5
CC(1/2)	0.997	0.997	0.314

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	10% (v/v) MPD 0.1 M MES pH 6.0