6T5Z

Crystal structure of an AA10 LPMO from Photorhabdus luminescens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	76.530, 80.390, 90.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.547 - 1.600
R-factor	0.189634216194
Rwork	0.188
R-free	0.21807
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bem
RMSD bond length	0.006
RMSD bond angle	0.881
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.550	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.078	0.547
Number of reflections	72679	7343
<I/σ(I)>	9.5	2.1
Completeness [%]	95.2	99.7
Redundancy	3.6	3.6
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	294	precipitant: 0.1 M sodium acetate (pH 5.2), 0.7 M 1,6-hexanediol protein solution: holo-PlAA10 (10 mg/mL) in 50 mM MES (pH 6.5), 0.5 mM CuSO4