6T48
Bovine enterovirus F3 in complex with glutathione and a Cysteinylglycine dipeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 344.000, 349.400, 352.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.170 |
| Rwork | 0.200 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5osn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.374 | 0.798 |
| Rpim | 0.187 | 0.787 |
| Number of reflections | 908128 | 37002 |
| <I/σ(I)> | 5.7 | 3.6 |
| Completeness [%] | 82.6 | 68 |
| Redundancy | 3.7 | |
| CC(1/2) | 0.275 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5 M Ammonium Sulfate and 0.1 M Tris at pH 8.5 |
