6T40
Bovine enterovirus F3 in complex with a Cysteinylglycine dipeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 342.700, 348.300, 351.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.670 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5osn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.216 | |
| Rmeas | 0.234 | |
| Rpim | 0.089 | |
| Number of reflections | 2361113 | 112481 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.4 | 95.4 |
| Redundancy | 6.9 | 5.2 |
| CC(1/2) | 0.994 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5 M Ammonium Sulfate, 0.1 M Tris at pH 8.5 |
