6T3O
Crystal structure of the human myomesin domain 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-30 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.599, 58.599, 62.821 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.850 - 1.800 |
| R-factor | 0.20471 |
| Rwork | 0.198 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y23 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.995 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.850 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.025 | 0.572 |
| Rmeas | 0.031 | 0.706 |
| Rpim | 0.017 | 0.408 |
| Number of reflections | 10591 | 3310 |
| <I/σ(I)> | 2.5 | 2.5 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.2 | 5.4 |
| CC(1/2) | 1.000 | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 291 | 0.1 M citrate/phosphate buffer pH 5.4, 30 % PEG3350 |
