6T3A
Difference-refined structure of rsEGFP2 10 ns following 400-nm laser irradiation of the off-state determined by SFX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2015-07-25 |
| Detector | MPCCD |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.680, 62.890, 71.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.300 - 1.850 |
| Rwork | 0.233 |
| R-free | 0.28670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dty |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.510 |
| Data reduction software | CrystFEL (0.6.2) |
| Data scaling software | CrystFEL (0.6.2) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.300 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 18058 | 1431 |
| <I/σ(I)> | 7.99 | |
| Completeness [%] | 99.8 | 98.14 |
| Redundancy | 303 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 293 | 100 mM HEPES pH 8.0, 2.5 M ammonium sulphate |
