6T39
Crystal structure of rsEGFP2 in its off-state determined by SFX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2015-07-25 |
| Detector | MPCCD |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.680, 62.890, 71.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.460 - 1.600 |
| Rwork | 0.165 |
| R-free | 0.20240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dty |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.464 |
| Data reduction software | CrystFEL (0.6.2) |
| Data scaling software | CrystFEL (0.6.2) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.460 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 31252 | 1832 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.9 | 88.25 |
| Redundancy | 235 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 293 | 100 mM HEPES pH 8.0, 2.5 M ammonium sulphate |
