6T2J
dAb3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.740, 59.309, 63.846 |
| Unit cell angles | 90.00, 131.97, 90.00 |
Refinement procedure
| Resolution | 43.420 - 1.700 |
| R-factor | 0.1767 |
| Rwork | 0.175 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ohq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.038 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.420 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.074 | 0.384 |
| Number of reflections | 26230 | 2586 |
| <I/σ(I)> | 11.36 | |
| Completeness [%] | 99.8 | 99.35 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Ammonium dihydrogen phosphate, Tris |
