6T1W

Structure of E. coli BamA in complex with lipoprotein RcsF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.77
Spacegroup name	C 1 2 1
Unit cell lengths	158.840, 142.530, 116.420
Unit cell angles	90.00, 102.61, 90.00

Refinement procedure

Resolution	48.580 - 3.790
R-factor	0.283
Rwork	0.281
R-free	0.31700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d0o
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	STARANISO (v3.309)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.580	3.993
High resolution limit [Å]	3.790	3.791
Rpim	0.020	0.788
Number of reflections	18489	897
<I/σ(I)>	15.8
Completeness [%]	92.8	24.6
Redundancy	34
CC(1/2)	0.990	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.03 M sodium nitrate, 0.03 M sodium phosphate dibasic, 0.03 M ammonium sulfate, 0.10 M Tris-base [pH 8.5]; BICINE, 20 % (v/v) ethylene glycol; 10 % w/v PEG 8000