6T1H
OXA-51-like beta-lactamase OXA-66
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-25 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 87.544, 87.544, 90.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.772 - 2.100 |
| R-factor | 0.1915 |
| Rwork | 0.188 |
| R-free | 0.22360 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.843 |
| Data reduction software | PROTEUM2 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (1.10) |
| Refinement software | PHENIX (1.10) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.060 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.165 | 0.760 |
| Rpim | 0.019 | 0.112 |
| Number of reflections | 21092 | 2689 |
| <I/σ(I)> | 3.75 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 75.4 | 45.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.1 M MES pH 6.5 22.85% (v/v) PEG MME 550 0.01 M ZnSO4 |
