6T1F
Crystal structure of the C-terminally truncated chromosome-partitioning protein ParB from Caulobacter crescentus complexed to the centromeric parS site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.250, 172.928, 72.852 |
| Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
| Resolution | 72.960 - 2.900 |
| R-factor | 0.2407 |
| Rwork | 0.239 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4umk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.045 |
| Data reduction software | XDS (Nov11-2017) |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 172.930 | 172.930 | 3.080 |
| High resolution limit [Å] | 2.900 | 8.700 | 2.900 |
| Rmerge | 0.135 | 0.057 | 1.526 |
| Rmeas | 0.146 | 0.062 | 1.646 |
| Rpim | 0.057 | 0.025 | 0.614 |
| Total number of observations | 198135 | 6755 | 33888 |
| Number of reflections | 29654 | 1130 | 4775 |
| <I/σ(I)> | 8.7 | 26.5 | 1.4 |
| Completeness [%] | 99.7 | 99.4 | 100 |
| Redundancy | 6.7 | 6 | 7.1 |
| CC(1/2) | 0.997 | 0.996 | 0.677 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | NULL |
